SNX1(Sorting nexin 1) is a member of a large family of hydrophilic proteins that interact with a variety of receptor types and are involved in intracellular trafficking. SNX1 and the related splice variant, SNX1A, bind the epidermal growth factor (EGF) receptor, facilitate its transport to lysosome, and thereby contribute to the degradation of the receptor. This protein is all partially associated with cellular membranes, and they, likewise, associate with EGF, PDGF and Insulin receptor tyrosine kinases. Recombinant human SNX1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.