SLAMF1, also known as signaling lymphocytic activation molecule family member 1, is a cell surface sialylated phosphoglycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. This protein is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. High-affinity for self-ligand is important in bidirectional T-cell to B-cell stimulation. Recombinant mouse SLAMF1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.