Septin2 (SEPT2) is a GTPase that is required for cytokinesis and associated with exocytosis. This protein can hetero-oligomerize with Septin6, 7 and also plays a role in the organization of new growth in organisms. It is associated with a Tau-based paired helical filament core and may contribute to the formation of neurofibrillary tangle as integral constituents of paired helical filaments. Recombinant human Septin2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.