SCGN, also known as secretagogin, is a cytoplasmic protein that contains six EF-hand domains and is related to the calicium-binding proteins Calretinin and Calbindin D28K. This protein is thought to be involved in cell proliferation and KCl (potassium chloride)-mediated calcium flux events. Through its interaction with KCl and its subsequent ability to modulate calcium storage pools within the cell, SCGN may function to negatively control growth and differentiation rates and, thus, indirectly inhibit cell replication. Recombinant SCGN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.