RSG1 plays a role in targeted membrane trafficking most probably at the level of vesicle fusion with membranes. This protein is involved in cilium biogenesis by regulating the transport of cargo proteins to the basal body and to the apical tips of cilia. Recombinant human RSG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.