Ras association domain-containing protein 3, also known as RASSF3, is a member of RASSF family. Members of the RASSF family contain a highly conserved Ras association domain (Ral GDS/AF-6 or RA) and function as Ras effectors/tumor suppressors. RASSF3 is a ubiquitously expressed protein found in normal and cancerous tissues. It contains an N-terminal RA domain and a coiled-coil SARAH domain. Three isoforms, namely RASSF3A, RASSF3B and RASSF3C, may exist for RASSF3 due to alternative splicing. RASSF3B and RASSF3C are shorter than RASSF3A and do not contain the RA and SARAH domain. Recombinant human RASSF3 protein, fused to His-tag at N-terminus, was expressed in E.coli.