RARRES2, as known as retinoic acid receptor responder protein 2, is a distant member of the Cystatin superfamily. Members of this superfamily contain at least two intra-chain disulfide bonds and an alpha-helical structure over a distance of about 100 amino acids. This protein was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. Also, it has been implicated in autocrine/paracrine signaling for adipocyte differentiation and also stimulation of lipolysis. Recombinant human RARRES2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.