S100A10 also known as S100 calcium binding protein A10 is a group of small Ca2+ binding proteins with molecular weight of 10-12 kDa. Intracellular S100 proteins usually function as modulators of their target proteins, and exert various roles such as regulation of protein phosphorylation, enzyme activity, Ca2+ homeostasis, cytoskeleton components, and transcription factors. Recent research suggests a role for S100A10 as a prognostic marker and potential therapeutic target in colorectal cancer. Recombinant mouse S100A10, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.