ppa, 1-176aa, E.coli, His tag, E.coli

Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion.
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基本信息

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货号 TP03478
规格 100 µg
宿主 E.coli
Accession
分子大小 21.9kDa (196aa), confirmed by MALDI-TOF
AP_Mol_Weight
Tag N-6His
蛋白序列 MGSSHHHHHHSSGLVPRGSHMSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDRFMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGEDAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAEAAKAEIVASFERAKNK
纯度 > 95% by HPLC
浓度 1mg/ml (determined by Bradford assay)
配方 Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
别名 inorganic pyrophosphatase
生物学活性
保存条件 4°C 短期保存 (1-2 周). 长期保存在 -20°C or -70°C. 避免反复冻融.
注意 仅用于科学研究, 不能用于疾病诊断.

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