PLAT, also known as plasminogen activator, tissue type, is serine protease secreted by many cell types that participate in biological processes, such as tissue restructuring, cell migration, and tumor metastasis. It used to dissolve coronary fibrin clots that are the proximal causes of acute myocardial infarction. It plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. Recombinant human PLAT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.