Pgk1 also known as Phosphoglycerate kinase 1 is an X-linked enzyme that plays a key role in the glycolytic pathway. Pgk1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. This protein catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant mouse Pgk1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.