Pfn1 also known as Profilin-1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals. Recombinant rat Pfn1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.