PCOLCE also known as procollagen C-endopeptidase enhancer 1. PCOLCE binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity and C-terminal processed part of PCPE (CT-PCPE) may have a metalloproteinase inhibitory activity. Recombinant human PCOLCE, fused to His-tag at N-terminus, was expressed in E.coli