NXPH1, also known as Neurexophilin-1, is one of at least four vertebrate neuropeptide-like secreted glycoproteins in the neurexophilin family. It encodes a secreted protein with a variable N-terminal domain, a highly conserved, N-glycosylated central domain, a short linker region, and a cysteine-rich C-terminal domain. This protein forms a very tight complex with alpha neurexins, a group of proteins that promote adhesion between dendrites and axons. Genetic deletion of NXPH1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show defects in motor coordination. Recombinant human NXPH1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.