NTS, as known as neurotensin/neuromedin N preproprotein, is a secreted tridecapeptide, which is widely distributed throughout the central nervous system, and may function as a neurotransmitter or a neuromodulator. It may be involved in dopamine-associated pathophysiological events, in the maintenance of gut structure and function, and in the regulation of fat metabolism. Neurotensin also exhibits antimicrobial activity against bacteria and fungi. Recombinant human NTS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.