nth, 1-211aa, E.coli, His tag, E.coli

Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
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基本信息

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货号 TP03213
规格 50 µg
宿主 E.coli
Accession
分子大小 25.7 kDa (231aa) confirmed by MALDI-TOF
AP_Mol_Weight
Tag N-6His
蛋白序列 MGSSHHHHHHSSGLVPRGSHMNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI
纯度 > 95% by HPLC
浓度 0.5 mg/ml (determined by Bradford assay)
配方 Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
别名 Endonuclease III, b1633, JW1625, DNA-(apurinic or apyrimidinic site) lyase
生物学活性
保存条件 4°C 短期保存 (1-2 周). 长期保存在 -20°C or -70°C. 避免反复冻融.
注意 仅用于科学研究, 不能用于疾病诊断.

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