Nrp1, also known as neuropilin-1, is a transmembrane glycoprotein that regulates axon guidance and angiogenesis. It acts as a receptor for two different extracellular ligands, class 3 semaphorins and specific isoforms of vascular endothelial growth factor. It is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. Recombinant rat Nrp1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.