Melanoregulin, also known as MREG, is thought to play a role in membrane fusion and in regulating the biogenesis of disk membranes of photoreceptor rods. MREG is expressed in photoreceptor cells as two isoforms due to alternative splicing. MREG interacts with Peripherin-2, a photoreceptor specific tetraspanin protein that is required to maintain normal cell structure during the renewal process of membrane fusion. Recombinant human MREG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.