MMP14, also known as matrix metalloproteinase-14, is a membrane-anchored zinc-binding endopeptidase that is expressed at the leading edge of various invasive carcinomas and promotes tumor cell invasion through degradation of the extracellular matrix. It plays an important role in extracellular matrix(ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha V. It is a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Recombinant human MMP14, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.