MAD2L1-binding protein, also known as MAD2L1BP, belongs to the MAD2L1BP family. This protein may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. Recombinant human MAD2L1BP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.