Acyl-protein thioesterase 2, also known LYPLA2, is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein may hydrolyze fatty acids from Sacylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Recombinant human LYPLA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.