LTA4H also known as Leukotriene A-4 hydrolase is a bifunctional enzyme which converts leukotriene A4 to leukotriene B4 and acts as an aminopeptidase. This enzyme belongs to the family of hydrolases, specifically those acting on ether bonds (ether hydrolases). It participates in arachidonic acid metabolism. Recombinant human LTA4H, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.