LASP1, also known as LIM and SH3 domain protein 1, plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types. Recombinant human LASP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.