Klk8, as known as kallikrein-8, is a member of the tissue kallikrein family. This protein is capable of degrading a number of proteins such as casein, fibrinogen, fibronectin and collagen type IV. Also, it cleaves L1CAM in response to increased neural activity and induces neurite outgrowth and fasciculation of cultured hippocampal neurons. It plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Recombinant mouse Klk8, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.