Interferon induces a variety of effects on target cells including antiviral, antiproliferative, and immunomodulatory activities. Interferon α-2b can either inhibit or enhance immune cell activity within the tightly regulated microenvironment of inflammation, depending upon the concentration of the cytokine and the activation stage of the cell. Interferon α-2b was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.