IL-1beta is an inflammatory cytokine that might modulate angiogenesis by directly interacting with vascular endothelial cells or enhancing the production of proangiogenic factors via paracrine control. IL-1beta stimulates endothelial cell migration and proliferation, adhesion-molecule expression, inflammatory mediator production, and leukocyte recruitment. IL-1beta is required for tumor growth, metastasis, and angiogenesis in several animal models. Recombinant mouse IL-1beta, fused to His tag, was expressed in E.coli and purified by using conventional chromatography techniques.