HSF2 binding protein (HSF2BP) associates with HSF2. The interaction occurs between the trimerization domain of HSF2 and the amino terminal hydrophilic region of HSF2BP that comprises two leucine zipper motifs. HSF2BP may therefore be involved in modulating HSF2 activation. Recombinant human HSF2BP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.