HSCB belongs to the hscB family and contains 1 J domain. This protein is expressed in lung, brain, stomach, spleen, ovary, testis, liver, muscle and heart and localized to mitochondria and cytoplasm. It may act as a co-chaperone in iron-sulfur cluster assembly in mitochondria, Interacts with ISCU and HSPA9. Recombinant human HSCB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.