Heat shock factor binding protein 1-like, also known as HSBP1L1, belongs to a family of eukaryotic proteins identified as nucleotide exchange factors for HSP 70, which exhibit varying degrees of compartment and species specificity. This gene expression is regulated primarily at the transcription level. Recombinant human HSBP1L1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.