HINT1, also known as Histidine triad nucleotide-binding protein 1, is a member of superfamily named for a near C-terminal HXHXHXX motif (H:Histidine, X:a hydrophobic amino acid) positioned at the α-phosphate of nucleotide substrates. This protein hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMPmorpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.