HDHD3, also known as haloacid dehalogenase-like hydrolase domain-containing protein 3, belongs to the HAD-like hydrolase superfamily. This family of hydrolase enzymes includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. Recombinant human HDHD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.