HAX1 is known to associate with HS1, a substrate of Src family tyrosine kinases. It also interacts with the product of PKD2, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with F-actin-binding protein, cortactin. It is associated with autosomal recessive severe congenital neutropenia, also known as Kostmann syndrome. Recombinant human HAX1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.