HMGN1, also known as non-histone chromosomal protein HMG-14, belongs to the HMGN family. Chromosomal protein HMGN1 binds to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. This protein may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Recombinant human HMGN1 protein was expressed in E.coli and purified by using conventional chromatography techniques.