GYPA, also known as glycophorin-A isoform 1, is the major transmembrane sialoglycoprotein of red blood cells. It has been shown to contribute to the expression of the MN and Wright blood group antigens, to act as a receptor for the malaria parasite Plasmodium falciparum and Sendai virus, and along with the anion transporter, band 3, may contribute to the mechanical properties of the red blood cell membrane. It expression is locally increased in carotid atherosclerotic lesions of symptomatic compared to asymptomatic patients. Recombinant human GYPA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.