GUSB, also known as beta-glucuronidase isoform 1, is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It includes heparin sulfate, chondroitin sulfate and hyaluronan. Mutations in the GUSB are linked to mucopolysaccharidosis type VII. GUSB plays an important role in the degradation of dermatan and keratin sulfates. Recombinant human GUSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.