GTF2F2, also known as TFIIF, is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity. Recombinant human GTF2F2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.