GIP, also known as glucose-dependent insulinotropic polypeptide, is an important insulin-releasing hormone of the enteroinsular axis that has a functional profile of possible therapeutic value for type 2 diabetes. This protein is an important incretin hormone released into the circulation from endocrine K-cells of the duodenum and jejunum after ingestion of food1. It was evaluated for their ability to elevate cellular cAMP production and stimulate insulin secretion. It also promotes plasma triglyceride clearance in response to oral fat loading. In liver, GIP has been shown to enhance insulin-dependent inhibition of glycogenolysis. Recombinant human GIP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.