Galanin peptides preproprotein, also known as GAL, is localized in brain pathways involved in both cognition and affect, and may inhibit learning and memory by inhibiting neurotransmitter release and neuronal firing rate. It modulates a variety of physiological processed including cognition/memory, sensory/pain processing, neurotransmitter/hormone secretion, and feeding behavior. GAL is upregulated in primary afferent and sympathetic neurones and may be involved in the development of sympathetic perineuronal baskets (“rings”) following nerve injury. Recombinant human GAL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.