GAGE2D, as known as G antigen 2D, belongs to a family of proteins organized in clustered repeats. They have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. The first GAGE nomenclature was based on identified mRNA sequences, but the high identity of the GAGE members made impossible to separate products of paralogous genes from polymorph products. Recombinant human GAGE2D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.