GABARAP, also known as gamma-aminobutyric acid receptor-associated protein, is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. Recombinant GABARAP protein was expressed in E.coli and purified by using conventional chromatography techniques.