FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus. Recombinant human FKBP14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.