Fructose-1,6-bisphosphatase 1(FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6- diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.