EPGN is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. This protein has been reported to have high mitogenic activity but low affinity for its receptor. Expression of this transcript and protein has been reported in cancer specimens of the breast, bladder, and prostate. Alternative splicing results in multiple transcript variants. Recombinant human EPGN, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.