Eukaryotic translation initiation factor 4E-binding protein 3, also known as EIF4EBP 3, is a member of EIF4EBP family which derives its name from proteins that bind to eukaryotic initiation factor 4E and that prevent its assembly into EIF4F. The EIF4F subunit EIF4E interacts directly with the mRNA 5' cap structure. Co-transcription of this gene and the neighboring upstream gene (MASK) generates a transcript (MASK-BP3) which encodes a fusion protein comprised of the MASK protein sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. Recombinant human EIF4EBP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.