Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border,like epithelial cells of the intestinal mucosa,gall bladder,renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein,and to have an important function in regulating actin dynamics,cell morphology,epithelial-to-mesenchymal transitions,cell migration and cell survival. In addition,VIL1 is a useful diagnostic marker for of various cancer,like cervical and endometrial adenocarcinomas,renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.